{"id":274,"date":"2019-07-02T17:53:44","date_gmt":"2019-07-02T09:53:44","guid":{"rendered":"http:\/\/wangliqiang.com\/?p=274"},"modified":"2019-07-02T17:53:44","modified_gmt":"2019-07-02T09:53:44","slug":"wb%e5%ae%9e%e9%aa%8c%e5%86%85%e5%8f%82%e9%80%89%e6%8b%a9%e7%9a%84%e5%8e%9f%e5%88%99","status":"publish","type":"post","link":"http:\/\/wangliqiang.com\/?p=274","title":{"rendered":"WB\u5b9e\u9a8c\u5185\u53c2\u9009\u62e9\u7684\u539f\u5219"},"content":{"rendered":"\n

\n\nWestern Blot\u5185\u53c2\u6297\u4f53\u79cd\u7c7b\u5f88\u591a\uff0c\u6bd4\u5982\u03b2-actin\u3001\u03b2-tubulin\u3001GAPDH\u3001Lamin B\u7b49\uff0c\u90a3\u4e48\u9488\u5bf9\u81ea\u5df1\u7684\u5b9e\u9a8c\uff0c\u6211\u4eec\u8be5\u5982\u4f55\u505a\u9009\u62e9\u5462?\u4e0b\u9762\u7b80\u5355\u4ecb\u7ecd\u4e0b\u9009\u62e9\u5185\u53c2\u6297\u4f53\u5e94\u9075\u5faa\u7684\u539f\u5219\uff1a 
<\/p>\n\n\n\n

> <\/strong>\u6837\u672c\u7684\u79cd\u5c5e\u6765\u6e90<\/strong><\/p>\n\n\n\n

        \u9009\u62e9\u5185\u53c2\u6297\u4f53\u9996\u5148\u8981\u8003\u8651\u7684\u662f\u5b9e\u9a8c\u6837\u672c\u7684\u6765\u6e90\u3002<\/p>\n\n\n\n

1.     \u54fa\u4e73\u52a8\u7269\u7684\u7ec4\u7ec7\u6216\u8005\u7ec6\u80de\u6837\u672c\uff0c\u901a\u5e38\u9009\u62e9\u03b2-actin\u3001\u03b2-tubulin\u3001GAPDH\u3001Lamin B\u3001Histone H3\u3001Na\/K ATPase\u7b49\u3002<\/p>\n\n\n\n

2.     \u690d\u7269\u6765\u6e90\u5b9e\u7684\u9a8c\u6837\u672c\uff0c\u5219\u53ef\u4ee5\u9009\u62e9plant actin\u3001Rubisco\u7b49\u3002<\/p>\n\n\n\n

3.     \u5176\u4ed6\u6765\u6e90\u6837\u672c\u7814\u7a76\u8f83\u5c11\uff0c\u6240\u4ee5\u7814\u7a76\u8005\u5c31\u9700\u8981\u53c2\u7167\u6587\u732e\u62a5\u5bfc\uff0c\u9009\u62e9\u5408\u9002\u7684\u6297\u4f53\u4f5c\u4e3a\u5185\u53c2\u3002<\/p>\n\n\n\n

><\/strong> \u76ee\u7684\u86cb\u767d\u7684\u5206\u5b50\u91cf\u5927\u5c0f<\/strong><\/p>\n\n\n\n

        \u9009\u62e9\u5185\u53c2\u6297\u4f53\u65f6\u5e94\u8be5\u8003\u8651\u76ee\u7684\u86cb\u767d\u5206\u5b50\u91cf\u7684\u5927\u5c0f\uff0c\u901a\u5e38\u5e94\u8be5\u4fdd\u8bc1\u76ee\u7684\u86cb\u767d\u4e0e\u5185\u53c2\u86cb\u767d\u5206\u5b50\u91cf\u76f8\u5dee5 kDa\u4ee5\u4e0a\u3002\u6bd4\u5982\u76ee\u7684\u86cb\u767d\u5206\u5b50\u91cf\u4e3a45 kDa\uff0c\u6b64\u65f6\u4e0d\u9002\u5b9c\u9009\u62e9\u03b2-actin\uff08\u5206\u5b50\u91cf\u4e3a42 kDa\uff09\u4f5c\u4e3a\u5185\u53c2\uff0c\u53ef\u4ee5\u8003\u8651\u9009\u62e9GAPDH(\u5206\u5b50\u91cf\u4e3a38 kDa)\u6216\u8005\u03b2-tubulin\uff08\u5206\u5b50\u91cf\u4e3a55 kDa\uff09\u4f5c\u4e3a\u5185\u53c2\u3002<\/p>\n\n\n\n

> <\/strong>\u76ee\u7684\u86cb\u767d\u7684\u8868\u8fbe\u90e8\u4f4d<\/strong>        \u4e00\u822c\u5b9e\u9a8c\u4e3b\u8981\u662f\u68c0\u6d4b\u80de\u6d46\u5185\u7684\u8868\u8fbe\u86cb\u767d\uff0c\u9009\u62e9\u03b2-actin\u3001\u03b2-Tubulin\u3001GAPDH\u7684\u6297\u4f53\u5c31\u53ef\u4ee5\u4e86\uff1b\u800c\u5bf9\u4e8e\u6838\u86cb\u767d\u7684\u5b9a\u91cf\uff0c\u7279\u522b\u662f\u6837\u672c\u6765\u6e90\u4e8e\u7ec6\u80de\u6838\u5185\u86cb\u767d\u65f6\uff0c\u9009\u62e9\u6070\u5f53\u7684\u6838\u86cb\u767d\u5185\u53c2\u5219\u66f4\u80fd\u4f53\u73b0\u5185\u90e8\u53c2\u7167\u7684\u4ef7\u503c\uff0c\u5e38\u7528\u7684\u6838\u5185\u53c2\u6297\u4f53\u6709Lamin A\u3001Lamin B\u3001Histone H3\uff0c\u9664\u6b64\u4e4b\u5916\uff0c\u5176\u5b83\u5e38\u89c1\u7684\u6838\u86cb\u767d\u5185\u53c2\u8fd8\u6709PCNA\u3001K70\u3001K80\u7b49\uff0c\u5728\u4e00\u4e9b\u6587\u732e\u62a5\u9053\u4e2d\uff0cErk2\u3001TATA binding protein(TBP)\u4ee5\u53cac-Jun\u3001c-Fos\u7b49\u90fd\u6709\u4f7f\u7528\uff1b\u5bf9\u4e8e\u819c\u86cb\u767d\u68c0\u6d4b\uff0c\u6700\u5e38\u7528\u7684\u5185\u53c2\u6297\u4f53\u4e3aNa\/K ATPase\uff1b\u5bf9\u4e8e\u7ebf\u7c92\u4f53\u86cb\u767d\u7684\u68c0\u6d4b\uff0c\u5e38\u7528VDAC1\u548cCOX IV\u4f5c\u4e3a\u5185\u53c2\u6297\u4f53\u3002<\/p>\n\n\n\n

        \u4ee5\u4e0a\u51e0\u6761\u539f\u5219\u53ea\u662f\u9488\u5bf9\u901a\u5e38\u60c5\u51b5\uff0c\u9700\u8981\u6ce8\u610f\u7684\u95ee\u9898\u662f\u2014\u2014\u5185\u53c2\u7684\u9009\u62e9\u8fd8\u9700\u8981\u8003\u8651\u5b9e\u9645\u7684\u5b9e\u9a8c\u73af\u5883\uff0c\u6bd4\u5982\u67d0\u4e9b\u7ec6\u80de\u7531\u4e8e\u7ec4\u7ec7\u7f3a\u6c27\u3001\u7cd6\u5c3f\u75c5\u7b49\u56e0\u7d20\u4f1a\u5bfc\u81f4GAPDH\u7684\u8868\u8fbe\u91cf\u589e\u9ad8\uff0c\u6b64\u65f6GAPDH\u5c31\u4e0d\u9002\u5408\u505a\u5185\u53c2\u4e86\uff1b\u518d\u6bd4\u5982\u5728\u6d89\u53ca\u7ec6\u80de\u589e\u6b96\u76f8\u5173\u8bd5\u9a8c\u4e2d\uff0cc-Jun\u7531\u4e8e\u81ea\u8eab\u8868\u8fbe\u53d8\u5316\u4e5f\u4e0d\u9002\u5408\u505a\u5185\u53c2\uff1b\u800c\u5728\u51cb\u4ea1\u5b9e\u9a8c\u65f6\uff0cTBP\u3001Lamin\u7b49\u4e5f\u4e0d\u9002\u5408\u4f5c\u4e3a\u5185\u53c2\u3002\u56e0\u6b64\u8bbe\u8ba1\u5b9e\u9a8c\u65b9\u6848\u65f6\u5e94\u8be5\u7efc\u5408\u8003\u8651\u591a\u65b9\u9762\u56e0\u7d20\u5e76\u67e5\u8be2\u76f8\u5173\u6587\u732e\uff0c\u5728\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u4e5f\u5e94\u8be5\u6ce8\u610f\u5982\u679c\u5185\u53c2\u8868\u8fbe\u51fa\u73b0\u5f02\u5e38\uff0c\u53ef\u80fd\u662f\u7531\u4e8e\u7279\u6b8a\u539f\u56e0\u5bfc\u81f4\uff0c\u56e0\u800c\u9700\u8981\u67e5\u9605\u76f8\u5173\u6587\u732e\u5e76\u66f4\u6362\u5176\u4ed6\u6297\u4f53\u4f5c\u4e3a\u5185\u53c2\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

Western Blot\u5185\u53c2\u6297\u4f53\u79cd\u7c7b\u5f88\u591a\uff0c\u6bd4\u5982\u03b2-actin\u3001\u03b2-tubulin\u3001GAPDH\u3001Lamin B\u7b49 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[31],"tags":[],"class_list":["post-274","post","type-post","status-publish","format-standard","hentry","category-protocol"],"_links":{"self":[{"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/posts\/274","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=274"}],"version-history":[{"count":1,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/posts\/274\/revisions"}],"predecessor-version":[{"id":275,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/posts\/274\/revisions\/275"}],"wp:attachment":[{"href":"http:\/\/wangliqiang.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=274"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=274"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=274"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}