{"id":248,"date":"2018-12-29T17:03:24","date_gmt":"2018-12-29T09:03:24","guid":{"rendered":"http:\/\/wangliqiang.com\/?p=248"},"modified":"2019-07-05T01:00:18","modified_gmt":"2019-07-04T17:00:18","slug":"mtt%e5%ae%9e%e9%aa%8c%e5%8e%9f%e7%90%86","status":"publish","type":"post","link":"http:\/\/wangliqiang.com\/?p=248","title":{"rendered":"MTT\u5b9e\u9a8c\u539f\u7406"},"content":{"rendered":"\n

\u5b9e\u9a8c\u539f\u7406\uff1a<\/p>\n\n\n\n

MTT\u7ec6\u80de\u589e\u6b96\u53ca\u7ec6\u80de\u6bd2\u6027\u68c0\u6d4b(MTT Cell\nProliferation and Cytotoxicity Assay )\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u7ec6\u80de\u589e\u6b96\u548c\u7ec6\u80de\u6bd2\u6027\u7684\u68c0\u6d4b\u3002<\/p>\n\n\n\n

MTT\u53ef\u4ee5\u88ab\u7ebf\u7c92\u4f53\u5185\u7684\u4e00\u4e9b\u8131\u6c22\u9176\u8fd8\u539f\u751f\u6210\u7ed3\u6676\u72b6\u7684\u6df1\u7d2b\u8272\u4ea7\u7269formazan\u3002\u5728\u7279\u5b9a\u6eb6\u5242\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u88ab\u5b8c\u5168\u6eb6\u89e3\u3002\u7136\u540e\u901a\u8fc7\u9176\u6807\u4eea\u53ef\u4ee5\u6d4b\u5b9a570nm\u6ce2\u957f\u9644\u8fd1\u7684\u5438\u5149\u5ea6\u3002\u7ec6\u80de\u589e\u6b96\u8d8a\u591a\u8d8a\u5feb\uff0c\u5219\u5438\u5149\u5ea6\u8d8a\u9ad8\uff1b\u7ec6\u80de\u6bd2\u6027\u8d8a\u5927\uff0c\u5219\u5438\u5149\u5ea6\u8d8a\u4f4e\u3002<\/p>\n\n\n\n

\u4fdd\u5b58\u6761\u4ef6\uff1a<\/p>\n\n\n\n

    4\u2103\u6216-20\u2103\u4fdd\u5b58\uff0cMTT\u9700\u907f\u5149\u4fdd\u5b58\uff0c\u4e00\u5e74\u6709\u6548\uff1bMTT\u914d\u5236\u6210\u6eb6\u6db2\u540e\u9700-20\u2103\u907f\u5149\u4fdd\u5b58\uff1bFormazan\u6eb6\u89e3\u6db2\u4e5f\u53ef\u4ee5\u5ba4\u6e29\u4fdd\u5b58\u3002<\/p>\n\n\n\n

\u5b9e\u9a8c\u6b65\u9aa4\uff1a<\/p>\n\n\n\n

1. MTT\u6eb6\u6db2\u7684\u914d\u5236\uff1a\u75285ml MTT\u6eb6\u5242\u6eb6\u89e325mg MTT\uff0c\u914d\u5236\u62105mg\/ml\u7684MTT\u6eb6\u6db2\u3002\u914d\u5236\u540e\u5373\u53ef\u4f7f\u7528\uff0c\u6216\u76f4\u63a5 -20\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u4e5f\u53ef\u4ee5\u6839\u636e\u9700\u8981\u9002\u5f53\u5206\u88c5\u540e-20\u2103\u907f\u5149\u4fdd\u5b58\u3002<\/p>\n\n\n\n

2. \u901a\u5e38\u7ec6\u80de\u589e\u6b96\u5b9e\u9a8c\u6bcf\u5b54\u52a0\u5165100\u5fae\u53472000\u4e2a\u7ec6\u80de\uff0c\u7ec6\u80de\u6bd2\u6027\u5b9e\u9a8c\u6bcf\u5b54\u52a0\u5165100\u5fae\u53475000\u4e2a\u7ec6\u80de(\u5177\u4f53\u6bcf\u5b54\u6240\u7528\u7684\u7ec6\u80de\u7684\u6570\u76ee\uff0c\u9700\u6839\u636e\u7ec6\u80de\u7684\u5927\u5c0f\uff0c\u7ec6\u80de\u589e\u6b96\u901f\u5ea6\u7684\u5feb\u6162\u7b49\u51b3\u5b9a)\u3002\u6309\u7167\u5b9e\u9a8c\u9700\u8981\uff0c\u8fdb\u884c\u57f9\u517b\u5e76\u7ed9\u4e880-10\u5fae\u5347\u7279\u5b9a\u7684\u836f\u7269\u523a\u6fc0\u3002<\/p>\n\n\n\n

3. \u6bcf\u5b54\u52a0\u516510\u5fae\u5347MTT\u6eb6\u6db2\uff0c\u5728\u7ec6\u80de\u57f9\u517b\u7bb1\u5185\u7ee7\u7eed\u5b75\u80b24\u5c0f\u65f6\u3002<\/p>\n\n\n\n

4. \u6bcf\u5b54\u52a0\u5165100\u5fae\u5347Formanzan\u6eb6\u89e3\u6db2\uff0c\u5728\u7ec6\u80de\u57f9\u517b\u7bb1\u5185\u518d\u7ee7\u7eed\u5b75\u80b2\u3002\u76f4\u81f3\u5728\u666e\u901a\u5149\u5b66\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u53d1\u73b0formazan\u5168\u90e8\u6eb6\u89e3\u3002\u901a\u5e3837\u2103\u5b75\u80b24\u5c0f\u65f6\u5de6\u53f3\uff0c\u7d2b\u8272\u7ed3\u6676\u4f1a\u5168\u90e8\u6eb6\u89e3\u3002\u5982\u679c\u7d2b\u8272\u7ed3\u6676\u8f83\u5c0f\u8f83\u5c11\uff0c\u6eb6\u89e3\u7684\u65f6\u95f4\u4f1a\u77ed\u4e00\u4e9b\u3002\u5982\u679c\u7d2b\u8272\u7ed3\u6676\u8f83\u5927\u8f83\u591a\uff0c\u6eb6\u89e3\u7684\u65f6\u95f4\u4f1a\u957f\u4e00\u4e9b\u3002<\/p>\n\n\n\n

5. \u5728570nm\u6d4b\u5b9a\u5438\u5149\u5ea6\u3002\u5982\u65e0570nm\u6ee4\u5149\u7247\uff0c\u53ef\u4ee5\u4f7f\u7528560-600nm\u7684\u6ee4\u5149\u7247\u3002<\/p>\n\n\n\n

\u6ce8\u610f\u4e8b\u9879\uff1a<\/p>\n\n\n\n

\u7531\u4e8e\u4f7f\u752896\u5b54\u677f\u8fdb\u884c\u68c0\u6d4b\uff0c\u5982\u679c\u7ec6\u80de\u57f9\u517b\u65f6\u95f4\u8f83\u957f\uff0c\u4e00\u5b9a\u8981\u6ce8\u610f\u84b8\u53d1\u7684\u95ee\u9898\u3002\u4e00\u65b9\u9762\uff0c\u7531\u4e8e96\u5b54\u677f\u5468\u56f4\u4e00\u5708\u6700\u5bb9\u6613\u84b8\u53d1\uff0c\u53ef\u4ee5\u91c7\u53d6\u5f03\u7528\u5468\u56f4\u4e00\u5708\u7684\u529e\u6cd5\uff0c\u6539\u52a0PBS\uff0c\u6c34\u6216\u57f9\u517b\u6db2\uff1b\u53e6\u4e00\u65b9\u9762\uff0c\u53ef\u4ee5\u628a96\u5b54\u677f\u7f6e\u4e8e\u9760\u8fd1\u57f9\u517b\u7bb1\u5185\u6c34\u6e90\u7684\u5730\u65b9\uff0c\u4ee5\u7f13\u89e3\u84b8\u53d1\u3002<\/p>\n\n\n\n

   \nMTT\u6eb6\u5242\u5728\u4f4e\u6e29\u60c5\u51b5\u4e0b\u4f1a\u51dd\u56fa\uff0c\u4f7f\u7528\u524d\u8bf7\u5ba4\u6e29\u653e\u7f6e\u621620-25\u2103\u6c34\u6d74\u6e29\u80b2\u7247\u523b\u81f3\u5168\u90e8\u878d\u89e3\u540e\u4f7f\u7528\u3002<\/p>\n\n\n\n

   \nMTT\u914d\u5236\u6210\u6eb6\u6db2\u540e\u4e3a\u9ec4\u8272\uff0c\u9700\u907f\u5149\u4fdd\u5b58\uff0c\u957f\u65f6\u95f4\u5149\u7167\u4f1a\u5bfc\u81f4\u5931\u6548\u3002\u5f53\u989c\u8272\u53d8\u4e3a\u7070\u7eff\u8272\u65f6\uff0c\u8bf7\u52ff\u4f7f\u7528\u3002MTT\u6eb6\u6db2\u57284\u2103\u3001\u51b0\u6d74\u7b49\u8f83\u4f4e\u6e29\u5ea6\u60c5\u51b5\u4e0b\u4f1a\u51dd\u56fa\uff0c\u53ef\u4ee520-25\u2103\u6c34\u6d74\u6e29\u80b2\u7247\u523b\u81f3\u5168\u90e8\u878d\u89e3\u540e\u4f7f\u7528\u3002<\/p>\n\n\n\n

Formazan\u6eb6\u89e3\u6db2\u7ed3\u51bb\u6216\u4ea7\u751f\u6c89\u6dc0\u65f6\u53ef\u4ee537\u2103\u6c34\u6d74\u5b75\u80b2\u4ee5\u4fc3\u8fdb\u6eb6\u89e3\uff0c\u5e76\u4e14\u5fc5\u987b\u5728\u5168\u90e8\u6eb6\u89e3\u5e76\u6df7\u5300\u540e\u4f7f\u7528\u3002<\/p>\n\n\n\n

\u672c\u8bd5\u5242\u76d2\u7684\u68c0\u6d4b\u4f9d\u8d56\u4e8e\u8131\u6c22\u9176\u50ac\u5316\u7684\u53cd\u5e94\uff0c\u6240\u4ee5\u8fd8\u539f\u5242(\u4f8b\u5982\u4e00\u4e9b\u6297\u6c27\u5316\u5242)\u4f1a\u5e72\u6270\u68c0\u6d4b\uff0c\u5982\u679c\u5f85\u68c0\u6d4b\u4f53\u7cfb\u4e2d\u5b58\u5728\u8f83\u591a\u7684\u8fd8\u539f\u5242\uff0c\u9700\u8bbe\u6cd5\u53bb\u9664\u3002<\/p>\n\n\n\n

    \u7528\u9176\u6807\u4eea\u68c0\u6d4b\u524d\u9700\u786e\u4fdd\u6bcf\u4e2a\u5b54\u5185\u6ca1\u6709\u6c14\u6ce1\uff0c\u5426\u5219\u4f1a\u5e72\u6270\u6d4b\u5b9a\u3002<\/p>\n\n\n\n

\u4e0e\u5176\u4ed6\u5b9e\u9a8c\u7684\u5bf9\u6bd4\uff1a<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n
\"\"<\/figure>\n","protected":false},"excerpt":{"rendered":"

\u5b9e\u9a8c\u539f\u7406\uff1a MTT\u7ec6\u80de\u589e\u6b96\u53ca\u7ec6\u80de\u6bd2\u6027\u68c0\u6d4b(MTT Cell Proliferation and Cytotox […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[31],"tags":[],"class_list":["post-248","post","type-post","status-publish","format-standard","hentry","category-protocol"],"_links":{"self":[{"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/posts\/248","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=248"}],"version-history":[{"count":2,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/posts\/248\/revisions"}],"predecessor-version":[{"id":253,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=\/wp\/v2\/posts\/248\/revisions\/253"}],"wp:attachment":[{"href":"http:\/\/wangliqiang.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=248"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=248"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/wangliqiang.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=248"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}